A Rôle of Nuclei in Oxidative Phosphorylation
نویسندگان
چکیده
منابع مشابه
A rôle of nuclei in oxidative phosphorylation.
Isolated mitochondria will catalyze many of the oxidative reactions observed with intact cells. However, such enzymatic activity may be only an approximation of the in vivo metabolism. Metabolic studies of reconstituted cells have utilized washed residues which represent simultaneously isolated cellular components (l), the recombination of cellular components in vitro (2), and special media whi...
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Uncouplers of oxidative phosphorylation in mitochondria inhibit the coupling between the electron transport and phosphorylation reactions and thus inhibit ATP synthesis without affecting the respiratory chain and ATP synthase (H(+)-ATPase). Miscellaneous compounds are known to be uncouplers, but weakly acidic uncouplers are representative because they show very potent activities. The most poten...
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was proportional to the acidity of the imidazole -NH group. Tetrachloro-2trifluoromethylbenzimidazole was the most active (50% uncoupling of oxidative phosphorylation at 7-9 x 10-8M, pK5.04). Nitro-substituted analogues were less active than predicted from pK considerations or from partition-coefficient measurements. 3. Introduction of an -NH2 or -CO2H substituent caused a loss of uncoupling ac...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1953
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)38459-x